Structure and Phylogenetic of Gnrh Genes of Hard-lipped Barb (Osteochilus hasselti C.V)

Abstract: The gonadotropin-releasing hormone is known and named for its role as the final common signaling
molecule used by the brain to regulate reproduction in all vertebrates. Two Genomic DNA GnRH and cDNAs
of Hard-lipped barb carp, namely GnRH-II and GnRH-III, were firstly cloned from the brain reverse transcriptionpolymerase
chain reaction (RT-PCR). The length of genomic DNA GnRH-II was 632 bp and cDNA GnRH-II was
253 bp, the length of genomic DNA GnRH-III was 486 bp and cDNA GnRH-II was 285 bp, respectively.
The GnRH-II precursors encoded cDNA consisted of 84 amino acids and GnRH-III precursors encoded cDNA
consisted of 95 amino acids, including a signal peptide, GnRH-II decapeptide and a GnRH-associated peptide
(GAP) linked by a Gly-Lys-Arg proteolytic site. Recently, genes encoding two GnRH forms in hard-lipped barb
have been discovered.

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